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Helicobacter pylori infection in a developing c...
59,00 € *
ggf. zzgl. Versand

There are several books on the subject of Helicobacter pylori infection. However, this book gives a picture of Helicobacter pylori infection in a developing country. Poor socio-economic conditions, overcrowding and lack of good quality drinking water are conducive to the spread of this infection. Self prescription is common in our population as the medications used for the treatment are freely available in pharmacies without proper prescriptions from doctors and are used by the public at large without proper indications. This leads to difficulty in the diagnosis and eradication of this infection. It is important to educate the masses about the perils of self-medication. Also, it is equally important to stress treating physician to confirm eradication of H. pylori infection following completion of treatment. It follows that bacteria itself though lacking virulent pattern develops mutations on exposure to oral antibiotics and has become resistant to commonly prescribed drug regimen. It describes a way to work out of this difficult situation by the concerned health care providers.

Anbieter: Dodax
Stand: 14.08.2020
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Helicobacter pylori : Rapid culture method
55,90 € *
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Despite the progress in control of the microbial agents of disease, they remain great and unpredictable threat to health and science throughout the world and antimicrobial drug resistance also becomes widely distributed. In this book one can find: Helicobacter pylori introduction, history, basic morphology, pathogenicity, internal organization and invitro effects of antimicrobial, identification of H.pylori, specimens, microscopy, growth, atmosphere for culture, selective, non selective media, transportation, handling of biopsies, biochemical characteristics, source of infection, animals and water as potential source and transmission, modification media to become rapid, selective and differential media for H.pylori, moringa and ginger history classification, phytochemistry, medicinal uses and pharmacological properties.

Anbieter: Dodax
Stand: 14.08.2020
Zum Angebot
Detection and Characterization of some pathogen...
74,90 € *
ggf. zzgl. Versand

Conventional bacterial indicators continue to be the gold standard useful in assessment of the water quality. However, the presences of viable but non culturable bacteria continue to cause serious outbreaks through using water contaminated by pathogens for drinking and/or recreational purposes. The aim of this work was to combine the culture method with PCR both monoplex and multiplex in order to determine the potential risk of examined water. Culture methods were used to detect bacterial indicators and selected pathogenic bacteria namely Escherichia coli O157:H7, Legionella spp. and Helicobacter pylori. Furthermore, the PCR was used to detect E. coli O157:H7 and a multiplex PCR was used to simultaneously detect the selected six virulence genes (flic, stx1, stx2, eae, rfbE and hly). One hundred seventy five different water samples were collected from Egypt. Water samples were collected from River Nile (Rossita Branch), the Mediterranean Sea, water drainage, hospital wastewater and groundwater. Legionella spp. and H. pylori were detected by culture methods on selective media in 25 and 33% of the examined water samples, respectively.

Anbieter: Dodax
Stand: 14.08.2020
Zum Angebot
Sensor Technology for Water Quality Monitoring
312,00 CHF *
ggf. zzgl. Versand

Two methods for the detection of important human pathogens, Cryptosporidium parvum and Helicobacter pylori, were investigated: a fiber optic biosensor, and real time PCR. The mechanism for specific detection in both methods is recognition of specific DNA sequences in the target organisms. The biosensor that was used, the Analyte 2000, was originally developed for the detection of chemicals. It utilizes a fiber optic wave guide that propagates an evanescent light wave of very specific wavelength. The light excites fluorescent molecules bound to the waveguide, but not in the bulk solution, which theoretically enhances signal while reducing background interference. Attempts to develop this system for the detection of DNA were not successful due to poor detection of the target molecules. An assay analogous to a sandwich immunoassay was designed for use on the Analyte 2000. Specific oligonucleotide probes were designed to bind to the waveguides via biotin-streptavidin interaction, and were used to capture the target DNA. Pure target DNA representing unique genes in the organisms were synthesized by PCR. Detection of captured DNA was then attempted using an oligonucleotide detection probe designed to bind to the target. Two detection systems were employed: an indirect signal amplification system based on biotin-tyramide deposition, or direct detection of fluorescent signal from Cy-5 molecules. In all experiments performed there was very little difference between the signal generated with or without the target molecules. Many experiments were conducted to attempt to identify reasons for the poor signal. Signal was only of any significance when target amplicons were internally labeled with Cy-5 by PCR. Real time PCR as a method to detect the pathogens was also investigated. Though the PCR technique itself is very rapid, DNA extraction and purification requires preparation time. Filtration of up to one liter of well water, followed by concentration and 'cleaning' Helicobacter pylori cells by immunomagnetic separation, was used to detect H. pylori seeded in a water source. Following cell lysis, the extracted DNA could be used directly in conventional PCR targeting the 16S rRNA gene to detect less than 265 cells per liter of water. DNA purification was not required for this level of detection. Initial studies to amplify lysed cells by real time PCR indicated that an incorrect product was made. When purified DNA was used for real time PCR, the correct product was produced from DNA representing as few as 100 cells.

Anbieter: Orell Fuessli CH
Stand: 14.08.2020
Zum Angebot
Helicobacter pylori infection in a developing c...
96,90 CHF *
ggf. zzgl. Versand

There are several books on the subject of Helicobacter pylori infection. However, this book gives a picture of Helicobacter pylori infection in a developing country. Poor socio-economic conditions, overcrowding and lack of good quality drinking water are conducive to the spread of this infection. Self prescription is common in our population as the medications used for the treatment are freely available in pharmacies without proper prescriptions from doctors and are used by the public at large without proper indications. This leads to difficulty in the diagnosis and eradication of this infection. It is important to educate the masses about the perils of self-medication. Also, it is equally important to stress treating physician to confirm eradication of H. pylori infection following completion of treatment. It follows that bacteria itself though lacking virulent pattern develops mutations on exposure to oral antibiotics and has become resistant to commonly prescribed drug regimen. It describes a way to work out of this difficult situation by the concerned health care providers.

Anbieter: Orell Fuessli CH
Stand: 14.08.2020
Zum Angebot
Detection and Characterization of some pathogen...
116,00 CHF *
ggf. zzgl. Versand

Conventional bacterial indicators continue to be the gold standard useful in assessment of the water quality. However, the presences of viable but non culturable bacteria continue to cause serious outbreaks through using water contaminated by pathogens for drinking and/or recreational purposes. The aim of this work was to combine the culture method with PCR both monoplex and multiplex in order to determine the potential risk of examined water. Culture methods were used to detect bacterial indicators and selected pathogenic bacteria namely Escherichia coli O157:H7, Legionella spp. and Helicobacter pylori. Furthermore, the PCR was used to detect E. coli O157:H7 and a multiplex PCR was used to simultaneously detect the selected six virulence genes (flic, stx1, stx2, eae, rfbE and hly). One hundred seventy five different water samples were collected from Egypt. Water samples were collected from River Nile (Rossita Branch), the Mediterranean Sea, water drainage, hospital wastewater and groundwater. Legionella spp. and H. pylori were detected by culture methods on selective media in 25 and 33% of the examined water samples, respectively.

Anbieter: Orell Fuessli CH
Stand: 14.08.2020
Zum Angebot
Helicobacter pylori infection in a developing c...
51,99 € *
ggf. zzgl. Versand

There are several books on the subject of Helicobacter pylori infection. However, this book gives a picture of Helicobacter pylori infection in a developing country. Poor socio-economic conditions, overcrowding and lack of good quality drinking water are conducive to the spread of this infection. Self prescription is common in our population as the medications used for the treatment are freely available in pharmacies without proper prescriptions from doctors and are used by the public at large without proper indications. This leads to difficulty in the diagnosis and eradication of this infection. It is important to educate the masses about the perils of self-medication. Also, it is equally important to stress treating physician to confirm eradication of H. pylori infection following completion of treatment. It follows that bacteria itself though lacking virulent pattern develops mutations on exposure to oral antibiotics and has become resistant to commonly prescribed drug regimen. It describes a way to work out of this difficult situation by the concerned health care providers.

Anbieter: Thalia AT
Stand: 14.08.2020
Zum Angebot
Sensor Technology for Water Quality Monitoring
175,99 € *
ggf. zzgl. Versand

Two methods for the detection of important human pathogens, Cryptosporidium parvum and Helicobacter pylori, were investigated: a fiber optic biosensor, and real time PCR. The mechanism for specific detection in both methods is recognition of specific DNA sequences in the target organisms. The biosensor that was used, the Analyte 2000, was originally developed for the detection of chemicals. It utilizes a fiber optic wave guide that propagates an evanescent light wave of very specific wavelength. The light excites fluorescent molecules bound to the waveguide, but not in the bulk solution, which theoretically enhances signal while reducing background interference. Attempts to develop this system for the detection of DNA were not successful due to poor detection of the target molecules. An assay analogous to a sandwich immunoassay was designed for use on the Analyte 2000. Specific oligonucleotide probes were designed to bind to the waveguides via biotin-streptavidin interaction, and were used to capture the target DNA. Pure target DNA representing unique genes in the organisms were synthesized by PCR. Detection of captured DNA was then attempted using an oligonucleotide detection probe designed to bind to the target. Two detection systems were employed: an indirect signal amplification system based on biotin-tyramide deposition, or direct detection of fluorescent signal from Cy-5 molecules. In all experiments performed there was very little difference between the signal generated with or without the target molecules. Many experiments were conducted to attempt to identify reasons for the poor signal. Signal was only of any significance when target amplicons were internally labeled with Cy-5 by PCR. Real time PCR as a method to detect the pathogens was also investigated. Though the PCR technique itself is very rapid, DNA extraction and purification requires preparation time. Filtration of up to one liter of well water, followed by concentration and 'cleaning' Helicobacter pylori cells by immunomagnetic separation, was used to detect H. pylori seeded in a water source. Following cell lysis, the extracted DNA could be used directly in conventional PCR targeting the 16S rRNA gene to detect less than 265 cells per liter of water. DNA purification was not required for this level of detection. Initial studies to amplify lysed cells by real time PCR indicated that an incorrect product was made. When purified DNA was used for real time PCR, the correct product was produced from DNA representing as few as 100 cells.

Anbieter: Thalia AT
Stand: 14.08.2020
Zum Angebot
Detection and Characterization of some pathogen...
65,99 € *
ggf. zzgl. Versand

Conventional bacterial indicators continue to be the gold standard useful in assessment of the water quality. However, the presences of viable but non culturable bacteria continue to cause serious outbreaks through using water contaminated by pathogens for drinking and/or recreational purposes. The aim of this work was to combine the culture method with PCR both monoplex and multiplex in order to determine the potential risk of examined water. Culture methods were used to detect bacterial indicators and selected pathogenic bacteria namely Escherichia coli O157:H7, Legionella spp. and Helicobacter pylori. Furthermore, the PCR was used to detect E. coli O157:H7 and a multiplex PCR was used to simultaneously detect the selected six virulence genes (flic, stx1, stx2, eae, rfbE and hly). One hundred seventy five different water samples were collected from Egypt. Water samples were collected from River Nile (Rossita Branch), the Mediterranean Sea, water drainage, hospital wastewater and groundwater. Legionella spp. and H. pylori were detected by culture methods on selective media in 25 and 33% of the examined water samples, respectively.

Anbieter: Thalia AT
Stand: 14.08.2020
Zum Angebot