Helicobacter pylori VacA and Calcium Signalling in T-cells ab 59.9 € als Taschenbuch: How Helicobacter pylori evade immune response; An insight of VacA effects on intracellular calcium signalling in T-cells. Aus dem Bereich: Bücher, Wissenschaft, Biologie,
Helicobacter pylori VacA and Calcium Signalling in T-cells ab 59.9 EURO How Helicobacter pylori evade immune response; An insight of VacA effects on intracellular calcium signalling in T-cells
This book summarizes the challenges of Isolation of full length vacuolating cytotoxin A protein from gram negetive bacterium Helicobacter pylori by known molecular biology techniques.Use of cloning and western blotting techniques split this protein into two subunits: P33 and P55. Our understanding of the basic nature and properties of VacA need to be extended for proper isolation of this Cytotoxin protein molecule in complete form.
More than 50% of the world's population harbor Helicobacter pylori in their upper gastrointestinal tract. H. pylori persistently infects gastric mucosa and is associated with several diseases including peptic ulcer disease and gastric carcinoma. One of the most thoroughly studied virulence factors produced by H. pylori is the Vacuolating Cytotoxin A (VacA).The protein binds to the host cells and is internalized. Inside the host cells, it causes "vacuole"-like membrane vesicles in the cytoplasm of gastric epithelial cells. Besides vacuolation, VacA exerts various other effects on target cells. VacA also forms membrane-embedded pores at the inner-mitochondrial membrane, resulting in mitochondrial dysfunction by cytochrome c release and apoptosis induction. VacA suppresses nuclear translocation of nuclear factor of activated T-cells (NFAT) resulting in down regulation of interleukin-2 (IL2) gene transcription to efficiently block proliferation of T-cells. This book underlines the results showing involvement of VacA in the modulation of intracellular calcium signalling and therefore will provide new insights that are required to understand how VacA inhibits T-cell proliferation.