Erscheinungsdatum: 13.07.2018, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: Molecular Pathogenesis and Signal Transduction by Helicobacter pylori, Auflage: Softcover reprint of the original 1st ed. 2017, Redaktion: Backert, Steffen // Tegtmeyer, Nicole, Verlag: Springer International Publishing, Sprache: Englisch, Rubrik: Allgemeinmedizin // Diagnostik, Therapie, Seiten: 376, Informationen: Paperback, Gewicht: 583 gr, Verkäufer: averdo
Erscheinungsdatum: 02.02.2017, Medium: Buch, Einband: Gebunden, Titel: Molecular Pathogenesis and Signal Transduction by Helicobacter pylori, Redaktion: Tegtmeyer, Nicole // Backert, Steffen, Verlag: Springer-Verlag GmbH // Springer International Publishing, Sprache: Englisch, Schlagworte: Gastroenterologie // Krebs // Krankheit // Tumor // Forschung // medizinisch // psychologisch // Heilkunde // Humanmedizin // Medizin // Biologie // Mikrobiologie // Mikroorganismus // Virologie // Virus // Onkologie // Radioonkologie // MEDICAL // Microbiology // Naturopathy // Mikrobiologie und Virologie // Komplementäre Therapien // Heilverfahren und Gesundheit // Vorklinische Medizin // Grundlagenwissenschaften, Rubrik: Allgemeinmedizin // Diagnostik, Therapie, Seiten: 347, Abbildungen: 5 schwarz-weiße Tabellen, Bibliographie, Reihe: Current Topics in Microbiology and Immunology (Nr. 400), Informationen: Book, Gewicht: 723 gr, Verkäufer: averdo
Molecular Pathogenesis and Signal Transduction by Helicobacter pylori ab 170.99 € als pdf eBook: . Aus dem Bereich: eBooks, Fachthemen & Wissenschaft, Medizin,
Molecular Pathogenesis and Signal Transduction by Helicobacter pylori ab 181.99 € als gebundene Ausgabe: 1st ed. 2017. Aus dem Bereich: Bücher, Wissenschaft, Medizin,
Molecular Pathogenesis and Signal Transduction by Helicobacter pylori ab 181.99 EURO 1st ed. 2017
Molecular Pathogenesis and Signal Transduction by Helicobacter pylori ab 170.99 EURO
During infection the human body interacts intimately with the infectious pathogen. It is this interaction that determines disease outcome. Upon infection the protein NF-kappaB acts as a central signal transmitter of the innate immune system. This protein has two main functions: the activation of genes important for inflammation as well as those that secure survival of the cell. This dual role provides a mechanistic link between infections and cancer development, as seen during infection with the carcinogenic bacterium Helicobacter pylori. Bacteria that develop intracellularly, such as Legionella pneumophila, depend on the survival of the host cell and can thus benefit from NF-kappaB activation. But how is NF-kappaB activated in these infections? And how is the activation terminated? The author introduces us to NF-kappaB signalling and then analyses NF-kappaB activation during infections with H. pylori or L. pneumophila. She establishes a new test system for high-throughput screenings and identifies new cellular factors important in NF-kappaB activation and termination. The identification of these factors broadens our understanding of innate immune signalling.
This volume reviews the current state of research concerning bacterial virulence factors and the infection biology of Helicobacter pylori , which is the leading cause of peptic ulcers and gastric cancer worldwide. The chapters include cutting-edge findings on this fascinating microbe and discuss the general strategies of H. pylori infection and persistence, news on important H. pylori virulence factors, crosstalk with the microbiota, hot novel models and signaling mechanisms, risk factors of gastric disease and stomach cancer, and the impact of H. pylori infection on non-gastric diseases. Written by internationally respected scientists, this book will appeal to clinicians, researchers and advanced students alike.
Two methods for the detection of important human pathogens, Cryptosporidium parvum and Helicobacter pylori, were investigated: a fiber optic biosensor, and real time PCR. The mechanism for specific detection in both methods is recognition of specific DNA sequences in the target organisms. The biosensor that was used, the Analyte 2000, was originally developed for the detection of chemicals. It utilizes a fiber optic wave guide that propagates an evanescent light wave of very specific wavelength. The light excites fluorescent molecules bound to the waveguide, but not in the bulk solution, which theoretically enhances signal while reducing background interference. Attempts to develop this system for the detection of DNA were not successful due to poor detection of the target molecules. An assay analogous to a sandwich immunoassay was designed for use on the Analyte 2000. Specific oligonucleotide probes were designed to bind to the waveguides via biotin-streptavidin interaction, and were used to capture the target DNA. Pure target DNA representing unique genes in the organisms were synthesized by PCR. Detection of captured DNA was then attempted using an oligonucleotide detection probe designed to bind to the target. Two detection systems were employed: an indirect signal amplification system based on biotin-tyramide deposition, or direct detection of fluorescent signal from Cy-5 molecules. In all experiments performed there was very little difference between the signal generated with or without the target molecules. Many experiments were conducted to attempt to identify reasons for the poor signal. Signal was only of any significance when target amplicons were internally labeled with Cy-5 by PCR. Real time PCR as a method to detect the pathogens was also investigated. Though the PCR technique itself is very rapid, DNA extraction and purification requires preparation time. Filtration of up to one liter of well water, followed by concentration and 'cleaning' Helicobacter pylori cells by immunomagnetic separation, was used to detect H. pylori seeded in a water source. Following cell lysis, the extracted DNA could be used directly in conventional PCR targeting the 16S rRNA gene to detect less than 265 cells per liter of water. DNA purification was not required for this level of detection. Initial studies to amplify lysed cells by real time PCR indicated that an incorrect product was made. When purified DNA was used for real time PCR, the correct product was produced from DNA representing as few as 100 cells.