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Rupareliya:PCR: A Sensitive and Rapid M
43,29 € *
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Erscheinungsdatum: 10/2012, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: PCR: A Sensitive and Rapid Method, Titelzusatz: Detection of Helicobacter Pylori Compared To Other Conventional Methods, Autor: Rupareliya, Jayesh // Patel, Jagdish, Verlag: LAP Lambert Academic Publishing, Sprache: Englisch, Rubrik: Biologie // Allgemeines, Lexika, Seiten: 80, Informationen: Paperback, Gewicht: 136 gr, Verkäufer: averdo

Anbieter: averdo
Stand: 05.12.2020
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Helicobacter pylori : Rapid culture method
55,99 € *
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Helicobacter pylori : Rapid culture method ab 55.99 € als Taschenbuch: and The antibacterial effect of Moringa and Ginger against H. pylori. Aus dem Bereich: Bücher, Wissenschaft, Biologie,

Anbieter: hugendubel
Stand: 05.12.2020
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PCR: A Sensitive and Rapid Method
48,99 € *
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PCR: A Sensitive and Rapid Method ab 48.99 € als Taschenbuch: Detection of Helicobacter Pylori Compared To Other Conventional Methods. Aus dem Bereich: Bücher, Wissenschaft, Biologie,

Anbieter: hugendubel
Stand: 05.12.2020
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Helicobacter pylori : Rapid culture method
55,90 € *
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Despite the progress in control of the microbial agents of disease, they remain great and unpredictable threat to health and science throughout the world and antimicrobial drug resistance also becomes widely distributed. In this book one can find: Helicobacter pylori introduction, history, basic morphology, pathogenicity, internal organization and invitro effects of antimicrobial, identification of H.pylori, specimens, microscopy, growth, atmosphere for culture, selective, non selective media, transportation, handling of biopsies, biochemical characteristics, source of infection, animals and water as potential source and transmission, modification media to become rapid, selective and differential media for H.pylori, moringa and ginger history classification, phytochemistry, medicinal uses and pharmacological properties.

Anbieter: Dodax
Stand: 05.12.2020
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PCR: A Sensitive and Rapid Method
49,00 € *
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Helicobacter pylori is currently implicated in the pathogenesis of various of digestive tract disorders and also a risk factor in gastric carcinomas. H. pylori infection is detected by various invasive & non invasive methods and each method has its own pits & falls. Out of all the techniques PCR is considered to be most rapid, accurate and sensitive method. The present study is an attempt to evaluate and compare the efficiency of all invasive techniques considering PCR as a Gold standard test. As part of long-term clinical studies, the PCR assay has the advantage of detecting low numbers of bacteria after successful or unsuccessful therapy or prior to relapse and will, we hope, permit the evaluation of the efficacy of various antimicrobial regimens.

Anbieter: Dodax
Stand: 05.12.2020
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Sensor Technology for Water Quality Monitoring
312,00 CHF *
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Two methods for the detection of important human pathogens, Cryptosporidium parvum and Helicobacter pylori, were investigated: a fiber optic biosensor, and real time PCR. The mechanism for specific detection in both methods is recognition of specific DNA sequences in the target organisms. The biosensor that was used, the Analyte 2000, was originally developed for the detection of chemicals. It utilizes a fiber optic wave guide that propagates an evanescent light wave of very specific wavelength. The light excites fluorescent molecules bound to the waveguide, but not in the bulk solution, which theoretically enhances signal while reducing background interference. Attempts to develop this system for the detection of DNA were not successful due to poor detection of the target molecules. An assay analogous to a sandwich immunoassay was designed for use on the Analyte 2000. Specific oligonucleotide probes were designed to bind to the waveguides via biotin-streptavidin interaction, and were used to capture the target DNA. Pure target DNA representing unique genes in the organisms were synthesized by PCR. Detection of captured DNA was then attempted using an oligonucleotide detection probe designed to bind to the target. Two detection systems were employed: an indirect signal amplification system based on biotin-tyramide deposition, or direct detection of fluorescent signal from Cy-5 molecules. In all experiments performed there was very little difference between the signal generated with or without the target molecules. Many experiments were conducted to attempt to identify reasons for the poor signal. Signal was only of any significance when target amplicons were internally labeled with Cy-5 by PCR. Real time PCR as a method to detect the pathogens was also investigated. Though the PCR technique itself is very rapid, DNA extraction and purification requires preparation time. Filtration of up to one liter of well water, followed by concentration and 'cleaning' Helicobacter pylori cells by immunomagnetic separation, was used to detect H. pylori seeded in a water source. Following cell lysis, the extracted DNA could be used directly in conventional PCR targeting the 16S rRNA gene to detect less than 265 cells per liter of water. DNA purification was not required for this level of detection. Initial studies to amplify lysed cells by real time PCR indicated that an incorrect product was made. When purified DNA was used for real time PCR, the correct product was produced from DNA representing as few as 100 cells.

Anbieter: Orell Fuessli CH
Stand: 05.12.2020
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Sensor Technology for Water Quality Monitoring
175,99 € *
ggf. zzgl. Versand

Two methods for the detection of important human pathogens, Cryptosporidium parvum and Helicobacter pylori, were investigated: a fiber optic biosensor, and real time PCR. The mechanism for specific detection in both methods is recognition of specific DNA sequences in the target organisms. The biosensor that was used, the Analyte 2000, was originally developed for the detection of chemicals. It utilizes a fiber optic wave guide that propagates an evanescent light wave of very specific wavelength. The light excites fluorescent molecules bound to the waveguide, but not in the bulk solution, which theoretically enhances signal while reducing background interference. Attempts to develop this system for the detection of DNA were not successful due to poor detection of the target molecules. An assay analogous to a sandwich immunoassay was designed for use on the Analyte 2000. Specific oligonucleotide probes were designed to bind to the waveguides via biotin-streptavidin interaction, and were used to capture the target DNA. Pure target DNA representing unique genes in the organisms were synthesized by PCR. Detection of captured DNA was then attempted using an oligonucleotide detection probe designed to bind to the target. Two detection systems were employed: an indirect signal amplification system based on biotin-tyramide deposition, or direct detection of fluorescent signal from Cy-5 molecules. In all experiments performed there was very little difference between the signal generated with or without the target molecules. Many experiments were conducted to attempt to identify reasons for the poor signal. Signal was only of any significance when target amplicons were internally labeled with Cy-5 by PCR. Real time PCR as a method to detect the pathogens was also investigated. Though the PCR technique itself is very rapid, DNA extraction and purification requires preparation time. Filtration of up to one liter of well water, followed by concentration and 'cleaning' Helicobacter pylori cells by immunomagnetic separation, was used to detect H. pylori seeded in a water source. Following cell lysis, the extracted DNA could be used directly in conventional PCR targeting the 16S rRNA gene to detect less than 265 cells per liter of water. DNA purification was not required for this level of detection. Initial studies to amplify lysed cells by real time PCR indicated that an incorrect product was made. When purified DNA was used for real time PCR, the correct product was produced from DNA representing as few as 100 cells.

Anbieter: Thalia AT
Stand: 05.12.2020
Zum Angebot