Erscheinungsdatum: 08.08.2017, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: Helicobacter pylori : Rapid culture method, Titelzusatz: and The antibacterial effect of Moringa and Ginger against H.pylori, Autor: Alkhidir, Abdulazeem Abdulsalam Ibrahim // Mohammed Elfatih Ournasseir, Khalid O Abosalif // Elhussain, Khalid Mohamed Khalid, Verlag: LAP Lambert Academic Publishing, Sprache: Englisch, Rubrik: Biologie // Allgemeines, Lexika, Seiten: 144, Informationen: Paperback, Gewicht: 231 gr, Verkäufer: averdo
Conventional bacterial indicators continue to be the gold standard useful in assessment of the water quality. However, the presences of viable but non culturable bacteria continue to cause serious outbreaks through using water contaminated by pathogens for drinking and/or recreational purposes. The aim of this work was to combine the culture method with PCR both monoplex and multiplex in order to determine the potential risk of examined water. Culture methods were used to detect bacterial indicators and selected pathogenic bacteria namely Escherichia coli O157:H7, Legionella spp. and Helicobacter pylori. Furthermore, the PCR was used to detect E. coli O157:H7 and a multiplex PCR was used to simultaneously detect the selected six virulence genes (flic, stx1, stx2, eae, rfbE and hly). One hundred seventy five different water samples were collected from Egypt. Water samples were collected from River Nile (Rossita Branch), the Mediterranean Sea, water drainage, hospital wastewater and groundwater. Legionella spp. and H. pylori were detected by culture methods on selective media in 25 and 33% of the examined water samples, respectively.
Despite the progress in control of the microbial agents of disease, they remain great and unpredictable threat to health and science throughout the world and antimicrobial drug resistance also becomes widely distributed. In this book one can find: Helicobacter pylori introduction, history, basic morphology, pathogenicity, internal organization and invitro effects of antimicrobial, identification of H.pylori, specimens, microscopy, growth, atmosphere for culture, selective, non selective media, transportation, handling of biopsies, biochemical characteristics, source of infection, animals and water as potential source and transmission, modification media to become rapid, selective and differential media for H.pylori, moringa and ginger history classification, phytochemistry, medicinal uses and pharmacological properties.
The presence of Helicobacter pylori in sewage water was confirmed, thus there is a reservoir for H. pylori outside of the human stomach. In aquatic environment, this bacterium transforms from the characteristic bacillary form to coccal or cocco-bacilli form, which is non culturable. However, Culturing this bacterium for the first time by the use of MDCS method took place. Here is a similarity between H. pylori found in sewage and those obtained from clinical biopsy and drinking water as indicated by RFLP technique raising the possibility for a fecal-oral route of transmission
The content of the present investigation was to formulate and evaluate floating-bioadhesive tablets of amoxicillin trihydrate (ATFBT) to provide local action in the treatment of Helicobacter Pylori (H. Pylori) by prolonging the gastric residence time. The FBT were prepared by direct compression method using combination of hydroxy propyl methyl cellulose (HPMC K4M) and chitosan (CH), carbopol (CP974P) and polymethacrylic acid (PMA) as release retardants and bioadhesive polymers, and sodium bicarbonate (NaHCO3) as a gas-former. The prepared tablets were evaluated for their physical characters such as drug content, in vitro buoyancy, swelling index, in vitro drug release and stability studies. Further, the bioadhesive strength (BS) was determined using porcine gastric mucosa. DSC studies indicate the compatibility of the drug and excipients used in the formulation. The optimized formulation (F7) showed floating lag time of 32 ± 2.7 sec, total floating time more than 12 h, BS of 1.86±0.14 N, swelling index of more than 3.5 and was found to be physically stable when stored at 40oC/75% RH for 3 months.
Helicobacter pylori is currently implicated in the pathogenesis of various of digestive tract disorders and also a risk factor in gastric carcinomas. H. pylori infection is detected by various invasive & non invasive methods and each method has its own pits & falls. Out of all the techniques PCR is considered to be most rapid, accurate and sensitive method. The present study is an attempt to evaluate and compare the efficiency of all invasive techniques considering PCR as a Gold standard test. As part of long-term clinical studies, the PCR assay has the advantage of detecting low numbers of bacteria after successful or unsuccessful therapy or prior to relapse and will, we hope, permit the evaluation of the efficacy of various antimicrobial regimens.
Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Medical scientists have found a new way to diagnose peptic ulcer by scrutinizing the colour, shape and coating of the human tongue. Indeed, medical experts have brought up evidence showing that this the analysis of the tongue should be done via acupuncture therapy. Therefore, in case of detection of the disease, peptic ulcer, the Chinese scientists have suggested to patients to get treatment from acupuncture treatment or herbal medicine therapy. Further research have shown that the tongue diagnosis techniques can also be used as a noninvasive auxiliary diagnostic method and as an indicator for clinical outcomes for patients with peptic ulcer disease. Medical scientists have explained that a human tongue which is invaded by the Helicobacter pylori infection and blood stasis within the tongue, indicates that the latter has already become a victim of stomach ulcer. The individuals who have received treatment acupunture therapy and herbal medicine therapy, usually possess a tongue with a healthy white color and sublingual vein engorgement. The multiple symptoms of peptic ulcer can be abdominal pain, internal bleeding, bloating, vomitting, melena, hematemesis...
An optimized Ethylcellulose microspheres of Clarithromycin were successfully prepared using emulsion/ solvent evaporation method and Con-A was successfully attached to the microspheres using carbodiimide method. Attachment of lectin to the Ethylcellulose microspheres significantly increased the mucoadhesiveness and also controls the release of Clarithromycin in simulated GI fluids. Conjugation aggregation assay suggesting that the lectin can be able to target in the treatment of Helicobacter pylori infection in peptic ulcer disease. Gamma scintigraphy study suggesting that Con-A lectin conjugated Ethylcellulose microspheres have gained considerable attention due to their ability to adhere to the mucus layer, as well as to release the drug in a controlled manner. It is concluded that designed targeted delivery system could possibly increases the bioavailability of Clarithromycin and treated the gastric ulcer in an effective manner.
BACKGROUND:Chronic bacterial infection may be a causative agent that explains part of the link between childhood poverty and adult coronary heart disease. Hence, search for new risk factors in the development of unstable angina especially the infections like helicobacter pylori . Most of the studies showed either inconsistent association between unstable angina and Helicobacter pylori infection . Hence, we are justified in conducting a study to know the association between unstable angina and Helicobacter pylori infection. METHODS:30 patients admitted in Kasturba medical hospitals admitted with the history of unstable angina were evaluated for helicobacter pylori infection by the serological test of IgG H.Pylori by ELISA method. 30 age and sex matched controls were studied and evaluated by the similar method. RESULTS:Out of 30 cases, 15 were positive for IgG H.Pylori (50%) and out of 30 controls 14 were positive for H.Pylori (46.66%). The P value was found to be 0.79. Hence, it was not statistically significant. CONCLUSION:Based on our small study we found that there is no association between Unstable angina and Helicobacter pylori infection.