Insight into Host Adaptation of Enterohepatic Helicobacter Bacteria ab 78.99 € als Taschenbuch: A molecular study of the response of Helicobacter hepaticus to bile and the effect of Helicobacter bilis on human hepatoma cells. Aus dem Bereich: Bücher, Wissenschaft, Biologie,
Insight into Host Adaptation of Enterohepatic Helicobacter Bacteria ab 78.99 EURO A molecular study of the response of Helicobacter hepaticus to bile and the effect of Helicobacter bilis on human hepatoma cells
This book summarizes the challenges of Isolation of full length vacuolating cytotoxin A protein from gram negetive bacterium Helicobacter pylori by known molecular biology techniques.Use of cloning and western blotting techniques split this protein into two subunits: P33 and P55. Our understanding of the basic nature and properties of VacA need to be extended for proper isolation of this Cytotoxin protein molecule in complete form.
Understanding the physiology of bacterial pathogens is essential to design more effective therapies. Thus the biochemical and molecular characteristics of aspartate carbamoyltransferase, the first step in pyrimidine biosynthesis, was investigated in the human pathogen Helicobacter pylori. Using enzymatic analysis procedures including NMR spectroscopy and radioactive tracer analysis, the regulatory properties of H. pylori ACTase were determined. The results indicated that the first step in this pathway of H. pylori was highly regulated. To determine whether ACTase plays an essential role in cell survival in H. pylori, an attempt was made to construct a mutant deficient in this enzyme activity. However, no mutants were isolated. These findings suggest ACTase is essential for survival of H. pylori. As ACTase appeared to be a potential therapeutic target, the effects of PALA, a potent inhibitor of this enzyme, was tested on cell viability. However, PALA had no effect on the viability of the cells. The results suggested H. pylori transformed the inhibitor into non-cytotoxic products, thus providing the bacterium with a mechanism of resistance that appears unique.
I am deeply thankful to GOD by the grace of whom the present work was realized. To my family, whose vision, enthusiasm, and unswerving support helped to make a lifelong dream come true. Several approaches are currently utilized in the prolongation of the gastric residence time, including floating drug delivery systems (FDDS), also known as hydrodynamically balanced systems (HBS). Therefore, the aim of this work was to formulate the metronidazole as it has an important role in the treatment of Helicobacter pylori infection of the stomach in different controlled release forms to prolong its duration of action using the floating technique to target the drug into its site of action. In addition, to maintain effective localized concentration of metronidazole and improve patient compliance.
Enterohepatic Helicobacter species are emerging infectious disease agents. Infection of the enterohepatobiliary tract of several mammals by these bacteria results in various pathological disorders. The adaptation of Helicobacter hepaticus to human factors such as bile was investigated using proteomics and transcriptomics. Ninety-one different proteins were identified in the responses of H. hepaticus to human, porcine and bovine bile. These proteins participate in several key cellular processes including DNA replication, protein transcription, translation and folding, oxidative stress response, motility, virulence, and metabolism. In particular, the bacteria deployed several strategies such as inhibition of the TCA cycle and the electron transport chain as well as iron sequestration to ensure control of the levels of hydroxyl radicals. The responses of human HEp-2 and Huh7-derived cell-lines to H. hepaticus and Helicobacter bilis, respectively, were also investigated.
The central role of RNA in many cellular processes has created a high interest in the specific tasks RNA molecules and protein: RNA complexes perform in a cell. In this publication up-to-date experimental methods and protein:RNA interaction protocols were used to identify the native RNA binding motive and to characterize the key role of the protein ribosomal binding factor A(RbfA). To gain insights into the binding mode of RbfA toits target RNA, two RbfA constructs from Thermotoga maritima and Helicobacter pylori were employed. Comparison of the RbfA proteins with the published homologous structure from Escherichia coli, led to studies concerning the structural attributes between proteins from thermophilic and mesophilic systems. The different chapters detail the biophysical and biochemical methods used to investigatethe protein and protein: RNA structures and their function and the advances in understanding the biological processes in which the RbfA protein is involved in.
High Quality Content by WIKIPEDIA articles! Xanthomonas oryzae is a species of proteobacteria. The major host of the bacteria is rice. The species contains two pathovars which are non-European: Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. Oryzicola. Host resistance gene, Xa21,from Oryza longistaminata is integrated into the genome of Oryza sativa for the board range resistance of rice blight disease caused by Xanthomonas oryzae pv. Oryzae. The Proteobacteria are a major group (phylum) of bacteria. They include a wide variety of pathogens, such as Escherichia, Salmonella, Vibrio, Helicobacter, and many other notable genera.
This volume details our current understanding of the architecture and signaling capabilities of known canonical and non-canonical inflammasome complexes and highlights their action, in particular in response to infection with important bacterial model organisms and the corresponding disease pathologies. The first chapters review new insights into the assembly and structures of inflammasome components and emphasize general strategies of up- and downstream signaling events. In addition, the authors specifically discuss the composition and activity of inflammasomes during infection with various gut pathogens ( Salmonella , Shigella , Yersinia , Listeria and Helicobacter ), respiratory pathogens ( Mycobacterium , Legionella , Burkholderia and Streptococcus ) as well as skin and soft tissue pathogens ( Francisella and Staphylococcus ). The discoveries presented provide a better understanding of the cellular and molecular biology of inflammasomes, which will pinpoint important new therapeutic targets for the treatment and prevention of multiple infectious diseases in the future. It is a valuable resource for students, scientists and clinicians, providing up-to-date information on this emerging research topic.