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Insight into Host Adaptation of Enterohepatic H...
78,99 € *
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Insight into Host Adaptation of Enterohepatic Helicobacter Bacteria ab 78.99 € als Taschenbuch: A molecular study of the response of Helicobacter hepaticus to bile and the effect of Helicobacter bilis on human hepatoma cells. Aus dem Bereich: Bücher, Wissenschaft, Biologie,

Anbieter: hugendubel
Stand: 19.10.2020
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Challenges In Isolation Of Full Length Vacuolat...
35,90 € *
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This book summarizes the challenges of Isolation of full length vacuolating cytotoxin A protein from gram negetive bacterium Helicobacter pylori by known molecular biology techniques.Use of cloning and western blotting techniques split this protein into two subunits: P33 and P55. Our understanding of the basic nature and properties of VacA need to be extended for proper isolation of this Cytotoxin protein molecule in complete form.

Anbieter: Dodax
Stand: 19.10.2020
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Nucleotide synthesis and regulation in a human ...
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Understanding the physiology of bacterial pathogens is essential to design more effective therapies. Thus the biochemical and molecular characteristics of aspartate carbamoyltransferase, the first step in pyrimidine biosynthesis, was investigated in the human pathogen Helicobacter pylori. Using enzymatic analysis procedures including NMR spectroscopy and radioactive tracer analysis, the regulatory properties of H. pylori ACTase were determined. The results indicated that the first step in this pathway of H. pylori was highly regulated. To determine whether ACTase plays an essential role in cell survival in H. pylori, an attempt was made to construct a mutant deficient in this enzyme activity. However, no mutants were isolated. These findings suggest ACTase is essential for survival of H. pylori. As ACTase appeared to be a potential therapeutic target, the effects of PALA, a potent inhibitor of this enzyme, was tested on cell viability. However, PALA had no effect on the viability of the cells. The results suggested H. pylori transformed the inhibitor into non-cytotoxic products, thus providing the bacterium with a mechanism of resistance that appears unique.

Anbieter: Dodax
Stand: 19.10.2020
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Metronidazole "Controlled Release Delivery Syst...
71,90 € *
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I am deeply thankful to GOD by the grace of whom the present work was realized. To my family, whose vision, enthusiasm, and unswerving support helped to make a lifelong dream come true. Several approaches are currently utilized in the prolongation of the gastric residence time, including floating drug delivery systems (FDDS), also known as hydrodynamically balanced systems (HBS). Therefore, the aim of this work was to formulate the metronidazole as it has an important role in the treatment of Helicobacter pylori infection of the stomach in different controlled release forms to prolong its duration of action using the floating technique to target the drug into its site of action. In addition, to maintain effective localized concentration of metronidazole and improve patient compliance.

Anbieter: Dodax
Stand: 19.10.2020
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Insight into Host Adaptation of Enterohepatic H...
79,00 € *
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Enterohepatic Helicobacter species are emerging infectious disease agents. Infection of the enterohepatobiliary tract of several mammals by these bacteria results in various pathological disorders. The adaptation of Helicobacter hepaticus to human factors such as bile was investigated using proteomics and transcriptomics. Ninety-one different proteins were identified in the responses of H. hepaticus to human, porcine and bovine bile. These proteins participate in several key cellular processes including DNA replication, protein transcription, translation and folding, oxidative stress response, motility, virulence, and metabolism. In particular, the bacteria deployed several strategies such as inhibition of the TCA cycle and the electron transport chain as well as iron sequestration to ensure control of the levels of hydroxyl radicals. The responses of human HEp-2 and Huh7-derived cell-lines to H. hepaticus and Helicobacter bilis, respectively, were also investigated.

Anbieter: Dodax
Stand: 19.10.2020
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Studies on Protein: RNA Complexes
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The central role of RNA in many cellular processes has created a high interest in the specific tasks RNA molecules and protein: RNA complexes perform in a cell. In this publication up-to-date experimental methods and protein:RNA interaction protocols were used to identify the native RNA binding motive and to characterize the key role of the protein ribosomal binding factor A(RbfA). To gain insights into the binding mode of RbfA toits target RNA, two RbfA constructs from Thermotoga maritima and Helicobacter pylori were employed. Comparison of the RbfA proteins with the published homologous structure from Escherichia coli, led to studies concerning the structural attributes between proteins from thermophilic and mesophilic systems. The different chapters detail the biophysical and biochemical methods used to investigatethe protein and protein: RNA structures and their function and the advances in understanding the biological processes in which the RbfA protein is involved in.

Anbieter: Dodax
Stand: 19.10.2020
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Xanthomonas Oryzae
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High Quality Content by WIKIPEDIA articles! Xanthomonas oryzae is a species of proteobacteria. The major host of the bacteria is rice. The species contains two pathovars which are non-European: Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. Oryzicola. Host resistance gene, Xa21,from Oryza longistaminata is integrated into the genome of Oryza sativa for the board range resistance of rice blight disease caused by Xanthomonas oryzae pv. Oryzae. The Proteobacteria are a major group (phylum) of bacteria. They include a wide variety of pathogens, such as Escherichia, Salmonella, Vibrio, Helicobacter, and many other notable genera.

Anbieter: Dodax
Stand: 19.10.2020
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Beneficial effect of Curcumin on Helicobacter p...
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Introduction: Peptic ulcer disease (PUD) is the erosion in lining of stomach or duodenum. Predominantly caused by Helicobacter pylori infection in about 50% of the world's population.which can colonize in the gastric antrum of all patients producing an extensive, noninvasive inflammatory reaction in the gastric mucosa.Methods: Fortey patients newly diagnosed endoscopically with peptic ulcer disease to be allocated into group 1 treated with standard H. pylori eradication triple therapy, and group 2 patients treated with curcumin (500 mg) capsules three times daily for 14 days as an adjuvant to standard triple therapy.Results: The result showed that the use of curcumin as adjuvant therapy produced highly significant improvement in healing efficacy which was significantly distinguished in duodenal ulcer patients compared to gastric ulcer groups 2 patients (p0.05), along with a highly significant reduction in pro-inflammatory IL-1beta level in group 2 patients (p0.01). Conclusion: This study revealed that addition of curcumin as adjuvant therapy produced improvement in ulcer healing efficacy, and controlled the inflammatory and oxidative stress process induced by H. pylori infection.

Anbieter: Dodax
Stand: 19.10.2020
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Helicobacter Species
171,19 € *
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Identifying Helicobacter infection as the leading cause of peptic ulcer disease and gastric cancer has dramatically altered the treatment of these disease states. Over the last several decades, scientists have come to understand that the interplay between the bacteria, the host, and the environment all contribute to the clinical outcome of infection. In Helicobacter Species: Methods and Protocols, expert researchers in the field detail many of the methods and which are now commonly used to study Helicobacter infection. These include protocols and methods that have evolved over time, and standards across the field have been established which are essential for optimal outcomes and to allow comparison of data across different laboratories. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Helicobacter Species: Methods and Protocols seeks to aid scientists in further study of this crucially important research into Helicobacter research.

Anbieter: Dodax
Stand: 19.10.2020
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