Erscheinungsdatum: 06.05.2015, Medium: Taschenbuch, Einband: Kartoniert / Broschiert, Titel: Isolation and Identification of Helicobacter pylori from Sewage, Titelzusatz: Isolation and Identification Helicobacter pylori, Autor: Al-Rubaee, Alaa // Al-Sulami, Amin // Al-Taee, Asaad, Verlag: LAP Lambert Academic Publishing, Sprache: Englisch, Rubrik: Biologie // Allgemeines, Lexika, Seiten: 148, Informationen: Paperback, Gewicht: 238 gr, Verkäufer: averdo
Helicobacter Pylori Research: From Bench to Bedside ab 181.99 € als gebundene Ausgabe: From Bench to Bedside. Auflage 2016. Aus dem Bereich: Bücher, Wissenschaft, Biologie,
Conventional bacterial indicators continue to be the gold standard useful in assessment of the water quality. However, the presences of viable but non culturable bacteria continue to cause serious outbreaks through using water contaminated by pathogens for drinking and/or recreational purposes. The aim of this work was to combine the culture method with PCR both monoplex and multiplex in order to determine the potential risk of examined water. Culture methods were used to detect bacterial indicators and selected pathogenic bacteria namely Escherichia coli O157:H7, Legionella spp. and Helicobacter pylori. Furthermore, the PCR was used to detect E. coli O157:H7 and a multiplex PCR was used to simultaneously detect the selected six virulence genes (flic, stx1, stx2, eae, rfbE and hly). One hundred seventy five different water samples were collected from Egypt. Water samples were collected from River Nile (Rossita Branch), the Mediterranean Sea, water drainage, hospital wastewater and groundwater. Legionella spp. and H. pylori were detected by culture methods on selective media in 25 and 33% of the examined water samples, respectively.
Helicobacter pylori cagPAI genes play an important role in pathogenesis, however little is known about their functions in isolates from Turkish patients. We aimed to evaluate the intactness and the effect of the cagPAI genes (cagT, cagM, cagE, cagA) and cagA EPIYA motifs on the AGS morphological changes and IL-8 induction. Isolates from gastritis, duodenal and gastric ulcer patients with intact and partially deleted cagPAI genes induced higher IL-8 secretion than those with complete deletions.Infection of AGS cells with isolates that possess intact cagPAI and EPIYA-ABC resulted in the formation of the hummingbird phenotype. The cagA positive isolates induced higher IL-8 secretion than cagA negative isolates. Isolates from DU patients with more than one EPIYA-C motif induced higher concentrations of IL-8 than those with EPIYA-ABC. In conclusion, the intactness of the cagPAI in our isolates from different patients was not conserved. An intact cagPAI was found to play an important role in the pathogenesis of DU but not GU or gastritis. The cagA gene, but not other cagPAI genes, was associated with the induction of IL-8 and the morphological changes of the AGS cells.
Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Rabeprazole is an antiulcer drug in the class of proton pump inhibitors. It was developed by Eisai Co. and is marketed by Janssen-Cilag as rabeprazole sodium under the brand names AcipHex in the US, Pariet in Japan and Canada, & RAZO (FGP), Rablet in India. Short-term treatment in healing and symptomatic relief of duodenal ulcers and erosive or ulcerative gastroesophageal reflux disease (GERD), maintaining healing and reducing relapse rates of heartburn symptoms in patients with GERD, treatment of daytime and nighttime heartburn and other symptoms associated with GERD, long-term treatment of pathological hypersecretory conditions, including Zollinger-Ellison syndrome and in combination with amoxicillin and clarithromycin to eradicate Helicobacter pylori.
The presence of Helicobacter pylori in sewage water was confirmed, thus there is a reservoir for H. pylori outside of the human stomach. In aquatic environment, this bacterium transforms from the characteristic bacillary form to coccal or cocco-bacilli form, which is non culturable. However, Culturing this bacterium for the first time by the use of MDCS method took place. Here is a similarity between H. pylori found in sewage and those obtained from clinical biopsy and drinking water as indicated by RFLP technique raising the possibility for a fecal-oral route of transmission